流感B病毒诊断试剂盒（快检方法）_甲乙流感试剂 -广州健仑生物科技有限公司

流感B病毒诊断试剂盒（快检方法）

广州健仑生物科技有限公司

广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、凯必利、广州创仑等主流品牌。

流感B病毒诊断试剂盒（快检方法）

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

欢迎咨询

欢迎咨询2042552662

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司
【市场部】 杨永汉

【】 
【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

操作步骤
1. 标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在*、第二孔中分别加标准品100 l，然后在*、第二孔中加标准品稀释液50 l，混匀；然后从*孔、第二孔中各取100 l分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50 l，混匀；然后在第三孔和第四孔中先各取50 l弃掉，再各取50 l分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50 l分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50 l，混匀后从第七、第八孔中分别取50 l加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50 l，混匀后从第九第十孔中各取50 l弃掉。（稀释后各孔加样量都为50 l，浓度分别为36ng/L，24 ng/L ，12 ng/L，6ng/L，3 ng/L）。
2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40 l，然后再加待测样品10 l（样品zui终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。
3. 温育：用封板膜封板后置37℃温育30分钟。
4. 配液：将20倍浓缩洗涤液用蒸馏水20倍稀释后备用。
5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。

Steps
1. Standard dilution and sample loading: Prepare standard wells 10 wells in the enzyme-coated plate, add 100 l each to the first and second wells, and then add standards to the first and second wells Then add 100 l from the first well and the second well to the third well and the fourth well, add 50 l standard diluent to the third and the fourth wells, and mix well; Then take out 50 l in each of the third and fourth wells, discard 50 l each and add them to the fifth and the sixth wells, respectively, and then add 50 l standard diluent to the fifth and the sixth wells, respectively; After mixing, take 50 l from each of the fifth and sixth wells and add them to the seventh and the eighth wells respectively, then add 50 l standard diluent in the seventh and the eighth wells respectively, Eight holes were added 50 l added to the ninth and tenth holes, and then in the ninth tenth hole standard diluent were added 50 l, after mixing from the ninth tenth hole each take 50 l discarded. (Each well was diluted to 50 l at a dilution of 36 ng / L, 24 ng / L, 12 ng / L, 6 ng / L, 3 ng / L).
2. Sample loading: Set blank holes (blank control wells without sample and enzyme reagent, the rest of the same operation), the sample hole. Add 40 l sample diluent to the sample well on the ELISA plate, then add 10 l test sample (5 times final sample dilution). Add samples will be added to the bottom of the ELISA plate hole, try not touch the hole wall, gently shaking to mix.
3. Incubation: Seal the plate with a sealing plate and incubate at 37 C for 30 minutes.
4. Dosage: 20 times concentrated cleaning solution diluted 20 times with distilled water reserve.
5. Wash: Carefully remove the sealing plate membrane, discard the liquid, dry it, fill each well with a washing solution, and let it stand for 30 seconds before it is discarded. Repeat 5 times and pat dry.