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描述B-27 添加剂是无血清添加剂，用于海马神经元和其他中枢神经系统（CNS）神经元的生长和保持其短期或长期活性。B-27 添加剂是50X 的液体，可以与Neurobasal 培养基或Neurobasal -A 培养基组合使用，用于神经细胞培养而不需要再添加饲养层细胞。
B-27 添加剂适和应用于大多数神经细胞培养。对于特别的应用，我们提供 其他配方的B-27 添加剂.
质量控制检测
B-27 添加剂 进行了18天妊娠的原代大鼠（Sprague Dawley） 的胚胎海马神经元生长检测。 通过了细菌和真菌的检测. B-27 也做了1X 浓度的内毒素水平测试。
仅供研究使用。除非另有说明，不得用于任何动物或人类治疗或诊断。详细说明常用规格 Cell Type:
Neuronal Cells
Product Size:10 ml
Classification:Serum-Free
Other Additives:Vitamin A,
Antioxidant Cocktail,
Insulin
Purity or Quality Grade:Cell Culture Grade
Green Features:Sustainable packaging
Shipping Condition:Dry Ice
Regulatory Statement:For Research Use Only. Not for use in diagnostic procedures.
引文和参考文献 （17） D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels. 作者Congar P; Bergevin A; Trudeau LE杂志J Neurophysiol （2002） 87:1046-1102产品用途Using a culture system in which isolated DAergic neurons establish self-innervating synapses （"autapses"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）. ID:PN64425 Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice. 作者Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;杂志Neuron （2002） 33:789-804产品用途Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37 C in a 5% CO2 humidified incubator. ID:PN47418 Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells. 作者Kabos Peter; Kabosova Andrea; Neuman Toomas;杂志J Biol Chem （2002） 277:8763-8769产品用途Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen） ID:PN63471 Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death. 作者Lesuisse Christian; Martin Lee J;杂志J Neurobiol （2002） 51:9-23产品用途A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. ID:PN60461 An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells. 作者Joseph-Bravo Patricia; P rez-Mart nez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;杂志Brain Res Brain Res Protoc （2002） 9:93-104产品用途Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties ID:PN62028更多..回到页首 按细胞系排列的引文 细胞系名称 有机体类型 组织 SH-Sy5Y Human Brain Fetal neurons Rat Brain Neurons （post-natal/adult） Rat Brain Neurons （superior cervical ganglia - SCG） Rat Brain Neurons （cortical） Mouse Other Neural Stem Cells Rat Brain 主营：生化试剂、抗体、重组蛋白、细胞生物、分子生物、蛋白研究、微生物、Elisa试剂盒、耗材、标准品、 标准溶液 abcam、ABI、Axygen、corning、信号转导抗体、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R D、Roche、santa、sigma、Thermo等进口品牌 以下为本公司常备现货产品
*部分 invitrogen 畅销原装产品
品牌 货号 名称 规格 invitrogen 13778-075 Lipofectamine RNAiMAX 0.75ml invitrogen 13778-150 Lipofectamine RNAiMAX 1.5ml invitrogen 11668-027 Lipofectamine 2000 Reagent 0.75ml invitrogen 11668-019 Lipofectamine 2000 Reagent 1.5ml invitrogen 15596-026 TRlzol Reagent 100ml invitrogen 15596-018 TRlzol Reagent 200ml invitrogen 10296-010 Trizol LS Reagent 100ml invitrogen 10296-028 Trizol LS Reagent 200ml invitrogen 17504-044 B-27（黄颜色） 10ml invitrogen 12587-010 B-27（黄颜色）不含维生素A 10ml invitrogen 17502-048 N-2 （粉红颜色） 5ml invitrogen 18064-014 Superscript II Reverse Transcriptase 10,000U invitrogen 18080-044 Superscript III Reverse Transcriptase 10,000U
第二部分 HyClone胎牛血清
品牌 货号 名称 规格 HyClone SH30370.03 Australian Standard Fetal Bovine Serum 500ml HyClone SH30406.02 FOETAL BOVINE SERUM 500ml HyClone SH30401.01 New Zealand Calf Serum 500ml HyClone SH30066.02 FetalClone II 100ml HyClone SH30088.03 STANDARD FETAL BOVINE SERUM 500ml HyClone SH30118.02 Newborn Bovine Calf Serum, U.S. Origin 100ml HyClone SH30070.02 FETAL BOVINE SERUM 100ml HyClone SH30087.02 COSMIC CALF SERUM 100ml HyClone SH30070.03E FETALBOVINESERUM CHARACTERIZSED 500ml 100ml HyClone SH30070.03 FETAL BOVINE SERUM 500ml HyClone SH30080.03 FetalClone I 500ml HyClone SH30084.03 FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN 500ml HyClone SH30396.03 FETAL BOVINE SERUM CHARACTERIZED 500ml HyClone SV30087.02 南美源胎牛血清 500ml HyClone sv30087.01 南美源胎牛血清 100ml HyClone SH30071.03 FETAL BOVINE SERUM 500ml HyClone SH30071.02 FETAL BOVINE SERUM 100ml HyClone SH30080.02 FetalClone I 100ml HyClone SH30070.02E FETAL BOVINE SERUM CHARACTERIZSED 100ml HyClone SH30079.02 FETAL BOVINE SERUM 100ml HyClone SH30068.02 CHARCOAL/DEXTRAN TREATED FBS，
活性炭灭活血清 100ml HyClone SH30068.03 CHARCOAL/DEXTRAN TREATED FBS，
活性炭灭活血清 500ml HyClone SH30066.03 FetalClone II 500ml HyClone SH30109.03 HyClone FetalClone III 500ml HyClone SH30088.03 STANDARD FETAL BOVINE SERUM 500ml HyClone SH30088.02 STANDARD FETAL BOVINE SERUM 100ml HyClone SH30079.03 FETAL BOVINE SERUM 500ml
第二部分 Gibco胎牛血清
品牌 货号 名称 规格 Gibco 12662-029 MSC QUALIFIED FBS 500ML 500ML Gibco 10438-026 FBS, HI 500ML 500ML Gibco 10439-024 FBS, ES CELL QUAL. USDA APPD. 500ML 500ML Gibco 10099-141 FBS, AUSTRALIAN ORIGIN 500ML 500ML Gibco 26400-044 FBS, DIALYZED, USA ORIGIN 500ML 500ML Gibco 26140-079 FBS QUALIFIED, USA ORIGIN 500ML 500ML Gibco 16141-079 FBS, ES CELL QUAL, USA ORIGIN 500ML 500ML Gibco 16140-071 FBS, HI 500ML 500ML Gibco 16000-044 FBS, CERT, USA ORIGIN 500ML 500ML Gibco C2027050 FBS, URUGUAYAN ORIGIN 500ML Gibco 12657-029 巴西胎牛 500ml
