聚乙烯亚胺(细胞培养)(Polyethyleneimine;PEI;Epomine)
将外源基因导入真核细胞是分子生物学研究中的一项重要技术。转基因载体主要分为病毒载体系统和非病毒载体系统。病毒载体系统转染效率高,是体内基因治疗的主要工具,但安全性存在隐患,且有免疫原性,体内不能反复应用,制备较复杂,而且所能装载的外源DNA大小有限,使其应用受到很大限制。非病毒载体是病毒载体的重要补充途径,一般认为安全性高,载体一般无免疫原性,而且是基于质粒的转染,易于组装[1-2]。目前已有的非病毒型载体介导基因转染的方法有裸DNA注射、磷酸钙介导、电转移法、阳离子脂质体及阳离子聚合物介导等。1995年,Boussif首次报道了聚乙烯亚胺(细胞培养)(polyethylenimine,PEI)可作为非病毒载体,其后,聚乙烯亚胺成为阳离子多聚物研究zui快的领域。为了研究PEI基因转染试剂的转染效果,本文通过聚乙烯亚胺(细胞培养)PEI转染表达绿色荧光蛋白的pEGFP质粒到293T细胞,以观察其效果,并测定了多种因素对转染效率的影响。
CAS Number: 9002-98-6
订购信息:
货号
产品名称
规格
25448-500
Polyethyleneimine, branched (50% soln. in water, MW 750,000)
500g
25414-2
Polyethyleneimine, Linear (MW ~100,000)
2g
25439-2
Polyethylenimine "Max", (Mw 160,000) - High Potency Linear PEI
2g
24885-2
Polyethylenimine "Max"?, (Mw 4,000*) - High Potency Linear PEI (Equivalent to Mw ~2,500 in Free Base Form) Polyethylenimine
2g
24765-2
Polyethylenimine "Max"?, (nominally Mw 40,000*) - High Potency Linear PEI (Equivalent to Mw 25,000 in Free Base Form)
2g
00618-500
Polyethylenimine, branched (30% soln. in water, MW 70,000)
500g
06088-100
Polyethylenimine, branched (MW 1,200)
100g
06088-500
Polyethylenimine, branched (MW 1,200)
500g
06089-100
Polyethylenimine, branched (MW 1,800)
100g
06089-500
Polyethylenimine, branched (MW 1,800)
500g
19850-100
Polyethylenimine, branched (MW 10,000)
100g
19850-500
Polyethylenimine, branched (MW 10,000)
500g
06090-100
Polyethylenimine, branched (MW 50,000-100,000)
100g
06090-500
Polyethylenimine, branched (MW 50,000-100,000)
500g
02371-100
Polyethylenimine, branched (MW 600)
100g
02371-500
Polyethylenimine, branched (MW 600)
500g
21903-10
Polyethylenimine, branched, permethylated, permethobromide
10g
24313-2
Polyethylenimine, Linear (MW 2,500)
2g
23966-2
Polyethylenimine, Linear (MW 25,000)
2g
24314-2
Polyethylenimine, Linear (MW 250,000)
2g